Journal: Biosensors

Article Title: Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers

doi: 10.3390/bios11040125

Figure Lengend Snippet: Illustration of the automatic optical sensing measurement system using our developed software program. ( A ) Optical alignment of the ball-lensed optical fiber (BLOF) through monitoring of the optical signal intensity. ( B ) Biosensor measurement of the shift in resonant wavelengths in a given time (0–20 min).

Article Snippet: The following are available online at https://www.mdpi.com/article/10.3390/bios11040125/s1 , Figure S1: Gel electrophoresis results for the detection of Coxiella burnetii DNA in clinical specimens from patients with Q fever, using end-point PCR, Table S1: Primer sequences used for the amplification of Coxiella burnetii DNA, Table S2: Simulation conditions of the ball-lensed optical fiber BLOF) with Zemax software.

Techniques: Software

Journal: Biosensors

Article Title: Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers

doi: 10.3390/bios11040125

Figure Lengend Snippet: Schematic representation of the ball-lensed optical fiber (BLOF). ( A ) Structure of the BLOF. ( B ) Simulated results of the BLOF using Zemax software.

Article Snippet: The following are available online at https://www.mdpi.com/article/10.3390/bios11040125/s1 , Figure S1: Gel electrophoresis results for the detection of Coxiella burnetii DNA in clinical specimens from patients with Q fever, using end-point PCR, Table S1: Primer sequences used for the amplification of Coxiella burnetii DNA, Table S2: Simulation conditions of the ball-lensed optical fiber BLOF) with Zemax software.

Techniques: Software

Journal: Biosensors

Article Title: Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers

doi: 10.3390/bios11040125

Figure Lengend Snippet: Optimization and validation of the ball-lensed optical fiber (BLOF) bio-optical sensor for clinical application, using Coxiella burnetii DNA and clinical samples. ( A ) Limit of detection of the bio-optical sensor assay for amplified C. burnetii DNA. The colors represent the amount of the target: 2.5 × 10 4 copies/reaction (black); 2.5 × 10 3 copies/reaction (gray with darker 75%); 2.5 × 10 2 copies/reaction (gray with darker 50%); and negative control (red). ( B ) Linear relationship between the wavelength shift and the target concentration in 25 min. ( C ) Bar chart showing the resonant wavelength shift results in 8 min per sample. The colors represent the detection of C. burnetii DNA (P, gray) in 9 blood samples from patients with Q fever and of no C. burnetii DNA (N, red) in 9 blood samples from patients with other febrile diseases. The red line indicates a cut off for positive and negative in 8 min. Error bars indicate the standard deviation of the mean.

Article Snippet: The following are available online at https://www.mdpi.com/article/10.3390/bios11040125/s1 , Figure S1: Gel electrophoresis results for the detection of Coxiella burnetii DNA in clinical specimens from patients with Q fever, using end-point PCR, Table S1: Primer sequences used for the amplification of Coxiella burnetii DNA, Table S2: Simulation conditions of the ball-lensed optical fiber BLOF) with Zemax software.

Techniques: Biomarker Discovery, Amplification, Negative Control, Concentration Assay, Standard Deviation